更新時(shí)間:2016-06-21
人小細(xì)胞肺癌細(xì)胞;NCI-H446,開(kāi)始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。
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人小細(xì)胞肺癌細(xì)胞;NCI-H446
形態(tài)特性 上皮樣
生長(zhǎng)特性 貼壁/懸浮生長(zhǎng),混合
特征特性人小細(xì)胞肺癌細(xì)胞;NCI-H446該細(xì)胞是1982年由Carney D和Gazdar AF等從一位小細(xì)胞肺癌患者的胸腔積液中建立的。細(xì)胞的原始形態(tài)并不具有小細(xì)胞肺癌特征。這個(gè)細(xì)胞株是小細(xì)胞肺癌的生化和形態(tài)學(xué)上的變種,表達(dá)神經(jīng)元*的烯醇酶和腦型肌酸激酶同工酶;左旋多巴脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達(dá)到可檢測(cè)水平。與正常細(xì)胞相比,該細(xì)胞c-myc DNA序列擴(kuò)增約20倍,RNA增加15倍。zui初傳代培養(yǎng)基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰島素、0.01 mg/ml轉(zhuǎn)鐵
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 1:3~1:9傳代;每周換液2~3次。
傳代情況 C5
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
白介素27w(IL27w)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 27 Working Designation (IL27w) X17630 96T Rattus norvegicus (Rat)
粒細(xì)胞巨噬細(xì)胞集落刺激因子(GMCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17631 96T Canis familiaris; Canine (Dog)
粒細(xì)胞巨噬細(xì)胞集落刺激因子(GMCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17632 96T Homo sapiens (Human)
粒細(xì)胞巨噬細(xì)胞集落刺激因子(GMCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17633 96T Mus musculus (Mouse)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17634 96T Bos taurus; Bovine (Cattle)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17635 96T Canis familiaris; Canine (Dog)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17636 96T Mus musculus (Mouse)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17637 96T Rattus norvegicus (Rat)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17638 96T Oryctolagus cuniculus (Rabbit)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17639 96T Bos taurus; Bovine (Cattle)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17640 96T Canis familiaris; Canine (Dog)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17641 96T Chicken (Gallus)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17642 96T Cavia (Guinea pig )
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17643 96T Sus scrofa; Porcine (Pig)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17644 96T Oryctolagus cuniculus (Rabbit)
干擾素γ(IFNγ)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17645 96T Rhesus monkey (Simian)
粒細(xì)胞集落刺激因子(GCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17646 96T Homo sapiens (Human)
粒細(xì)胞集落刺激因子(GCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17647 96T Mus musculus (Mouse)
粒細(xì)胞集落刺激因子(GCSF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17648 96T Rattus norvegicus (Rat)
膠質(zhì)細(xì)胞系來(lái)源神經(jīng)營(yíng)養(yǎng)因子(GDNF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17649 96T Mus musculus (Mouse)
膠質(zhì)細(xì)胞系來(lái)源神經(jīng)營(yíng)養(yǎng)因子(GDNF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17650 96T Rattus norvegicus (Rat)
核糖核酸酶T2(RNASET2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17651 96T Homo sapiens (Human)
核糖核酸酶T2(RNASET2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17652 96T Mus musculus (Mouse)
核糖核酸酶T2(RNASET2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17653 96T Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17654 96T Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17655 96T Capra hircus; Caprine (Goat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17656 96T Equus caballus; Equine (Horse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17657 96T Chicken (Gallus)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17658 96T Mus musculus (Mouse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17659 96T Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17660 96T Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17661 96T Oryctolagus cuniculus (Rabbit)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17662 96T Rhesus monkey (Simian)
Rho鳥(niǎo)嘌呤核苷酸交換因子25(ARHGEF25)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17663 96T Homo sapiens (Human)
Rho鳥(niǎo)嘌呤核苷酸交換因子25(ARHGEF25)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17664 96T Mus musculus (Mouse)
Rho鳥(niǎo)嘌呤核苷酸交換因子25(ARHGEF25)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17665 96T Rattus norvegicus (Rat)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17666 96T Homo sapiens (Human)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17667 96T Mus musculus (Mouse)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17668 96T Rattus norvegicus (Rat)
干擾素調(diào)節(jié)因子2結(jié)合蛋白2(IRF2BP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17669 96T Homo sapiens (Human)
干擾素調(diào)節(jié)因子2結(jié)合蛋白2(IRF2BP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17670 96T Mus musculus (Mouse)
成纖維細(xì)胞生長(zhǎng)因子結(jié)合蛋白3(FGFBP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17671 96T Homo sapiens (Human)
成纖維細(xì)胞生長(zhǎng)因子結(jié)合蛋白3(FGFBP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17672 96T Mus musculus (Mouse)
成纖維細(xì)胞生長(zhǎng)因子結(jié)合蛋白3(FGFBP3)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17673 96T Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子145(VEGF145)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Vascular Endothelial Growth Factor 145 (VEGF145) X17674 96T Homo sapiens (Human)
肝癌衍生生長(zhǎng)因子樣蛋白1(HDGFL1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17675 96T Homo sapiens (Human)
肝癌衍生生長(zhǎng)因子樣蛋白1(HDGFL1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17676 96T Mus musculus (Mouse)
肝癌衍生生長(zhǎng)因子樣蛋白1(HDGFL1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17677 96T Rattus norvegicus (Rat)
神經(jīng)元衍生神經(jīng)營(yíng)養(yǎng)因子(NDNF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Neuron Derived Neurotrophic Factor (NDNF) X17678 96T Homo sapiens (Human)
錨定蛋白重復(fù)域蛋白50(ANKRD50)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17679 96T Homo sapiens (Human)
錨定蛋白重復(fù)域蛋白50(ANKRD50)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17680 96T Mus musculus (Mouse)
錨定蛋白重復(fù)域蛋白50(ANKRD50)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17681 96T Rattus norvegicus (Rat)
對(duì)氧磷酶1(PON1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Paraoxonase 1 (PON1) X17682 96T Homo sapiens (Human)
D-二聚體(D2D)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for D-Dimer (D2D) X17683 96T Mus musculus (Mouse)
載脂蛋白A1(APOA1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Apolipoprotein A1 (APOA1) X17684 96T Mus musculus (Mouse)
肌原纖蛋白1(FBN1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Fibrillin 1 (FBN1) X17685 96T Homo sapiens (Human)
粘蛋白5B(MUC5B)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Mucin 5 Subtype B (MUC5B) X17686 96T Homo sapiens (Human)
Toll樣受體4(TLR4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Toll Like Receptor 4 (TLR4) X17687 96T Mus musculus (Mouse)
血清淀粉樣P物質(zhì)(SAP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Serum Amyloid P Component (SAP) X17688 96T Homo sapiens (Human)
巨噬細(xì)胞清道夫受體1(MSR1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Macrophage Scavenger Receptor 1 (MSR1) X17689 96T Homo sapiens (Human)
主要堿性蛋白(MBP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Major Basic Protein (MBP) X17690 96T Mus musculus (Mouse)
黑素瘤細(xì)胞黏附分子(MCAM)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Melanoma Cell Adhesion Molecule (MCAM) X17691 96T Mus musculus (Mouse)
載脂蛋白A4(APOA4)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Apolipoprotein A4 (APOA4) X17692 96T Homo sapiens (Human)
上皮型脂肪酸結(jié)合蛋白(FABP5)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Fatty Acid Binding Protein 5, Epidermal (FABP5) X17693 96T Homo sapiens (Human)
整合素β6(ITGβ6)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Integrin Beta 6 (ITGb6) X17694 96T Homo sapiens (Human)
膜聯(lián)蛋白A1(ANXA1)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Annexin A1 (ANXA1) X17695 96T Homo sapiens (Human)
嘌呤能受體P2X7(P2RX7)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) X17696 96T Homo sapiens (Human)
絲聚蛋白(FLG)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Filaggrin (FLG) X17697 96T Homo sapiens (Human)
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