更新時(shí)間:2016-06-15
小鼠胚胎成纖維細(xì)胞;MEF,開始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實(shí)驗(yàn)。
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小鼠胚胎成纖維細(xì)胞;MEF
形態(tài)特性 成纖維細(xì)胞樣
生長特性 貼壁生長
特征特性小鼠胚胎成纖維細(xì)胞;MEF取孕9天的615小鼠胚胎,去除腦、心臟等培養(yǎng)建立。該細(xì)胞可用作飼養(yǎng)層細(xì)胞,支持胚胎干細(xì)胞ES的生長并維持ES未分化的狀態(tài)。當(dāng)作為飼養(yǎng)層細(xì)胞時(shí),MEF需經(jīng)絲裂霉素C處理停止生長。建議作為ES細(xì)胞的飼養(yǎng)層時(shí),MEF不要超過6代。
培養(yǎng)條件 DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose) 10%CS
傳代方法 1:3傳代;3~4天1次。
傳代情況 P1
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
干擾素α5(IFNα5)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Alpha 5 (IFNa5) X17458 96T Homo sapiens (Human)
干擾素α5(IFNα5)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interferon Alpha 5 (IFNa5) X17459 96T Mus musculus (Mouse)
白介素1受體輔助蛋白樣蛋白2(IL1RAPL2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 1 Receptor Accessory Protein Like Protein 2 (IL1RAPL2) X17460 96T Homo sapiens (Human)
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白介素1受體輔助蛋白(IL1RAP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 1 Receptor Accessory Protein (IL1RAP) X17464 96T Mus musculus (Mouse)
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肝素結(jié)合性表皮生長因子(HBEGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) X17466 96T Mus musculus (Mouse)
肝素結(jié)合性表皮生長因子(HBEGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) X17467 96T Rattus norvegicus (Rat)
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絲切蛋白1(CFL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Cofilin 1, Non Muscle (CFL1) X17469 96T Homo sapiens (Human)
絲切蛋白1(CFL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Cofilin 1, Non Muscle (CFL1) X17470 96T Mus musculus (Mouse)
絲切蛋白1(CFL1)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Cofilin 1, Non Muscle (CFL1) X17471 96T Rattus norvegicus (Rat)
淋巴毒素β(LTβ)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Lymphotoxin Beta (LTb) X17472 96T Homo sapiens (Human)
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粒細(xì)胞趨化蛋白2(GCP2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Granulocyte Chemotactic Protein 2 (GCP2) X17475 96T Homo sapiens (Human)
粒細(xì)胞趨化蛋白2(GCP2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Granulocyte Chemotactic Protein 2 (GCP2) X17476 96T Mus musculus (Mouse)
粒細(xì)胞趨化蛋白2(GCP2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Granulocyte Chemotactic Protein 2 (GCP2) X17477 96T Rattus norvegicus (Rat)
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白介素21(IL21)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 21 (IL21) X17493 96T Rattus norvegicus (Rat)
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白介素26(IL26)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 26 (IL26) X17505 96T Homo sapiens (Human)
白介素32(IL32)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Interleukin 32 (IL32) X17506 96T Homo sapiens (Human)
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干擾素γ誘導(dǎo)單核因子(MIγ)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Monokine Induced By Interferon Gamma (MIg) X17509 96T Rattus norvegicus (Rat)
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轉(zhuǎn)化生長因子β3(TGFβ3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) CLIA Kit for Transforming Growth Factor Beta 3 (TGFb3) X17513 96T Mus musculus (Mouse)
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