更新時間:2016-06-16
人急性T淋巴細胞白血病細胞;Jurkat, Clone E6-1,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。
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人急性T淋巴細胞白血病細胞;Jurkat, Clone E6-1
形態特性 淋巴母細胞樣
生長特性 懸浮生長
特征特性人急性T淋巴細胞白血病細胞;Jurkat, Clone E6-1該細胞是Jurkat-FHCRC細胞株(Jurkat細胞株的衍生)的一個克隆。Jurkat細胞株來源于一個14歲男孩的外周血;經佛波酯和外源凝集素或抗T3單克隆抗體中的一種誘導后可產生大量IL-2(IL-2的產生需兩種類型的誘導劑);表達T細胞受體、CD3。
培養條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 維持細胞濃度在1×105~1×106viable cells/ml;2~3天換液1次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
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