更新時間:2016-06-16
CIN-1培養基基礎,年中放價銷售了,低價、*、優惠價供應,微生物培養基折扣價提供,BD培養基、Gibco培養基、Amresco培養基、OXOID培養基等國內外培養基,我們傾情提供。
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CIN-1培養基基礎 | Cepulodin Irgasan Novobiocin Agar | 250g | 用于分離小腸結腸炎耶爾森氏菌。 |
用途:用于分離小腸結腸炎耶爾森氏菌
CIN-1培養基基礎配方:(g/L) | |||||||||||||||||
胰蛋白胨 酵母浸粉 甘露醇 氯化鈉 去氧膽酸鈉 硫酸鎂 瓊脂 Irgsan 中性紅 結晶紫 氯化鍶 pH值7.5 ± 0.1 | 20.0 2.0 20.0 1.0 2.0 0.01 12.0 0.004 0.03 0.001 1.0 25℃ | ||||||||||||||||
原理: | |||||||||||||||||
胰蛋白胨和酵母浸粉提供氮源和微量元素;甘露醇為可發酵糖;去氧膽酸鈉和結晶紫抑制革蘭氏陽性菌;中性紅是pH指示劑;瓊脂是培養基的凝固劑。 | |||||||||||||||||
用法: | |||||||||||||||||
稱取本品 58.0g,加熱溶解于 1000ml 蒸餾水中,分裝三角瓶,每瓶 200ml,121℃高壓滅菌 15 分鐘,冷至 50℃ 左右時,加入一支過濾除菌的 CIN-1 添加劑(含頭孢菌素 3mg,新生霉素 0.5mg)混勻,傾入無菌平皿。 | |||||||||||||||||
質量控制: | |||||||||||||||||
在25℃培養24-48小時。
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