更新時間:2016-06-17
支原體肉湯培養基(不含瓊脂和酚紅),年中放價銷售了,低價、*、優惠價供應,微生物培養基折扣價提供,BD培養基、Gibco培養基、Amresco培養基、OXOID培養基等國內外培養基,我們傾情提供。
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支原體肉湯培養基(不含瓊脂和酚紅) | Mycoplasma Broth Medium | 250g | 用于支原體的增菌培養。 |
用途:用于支原體的增菌培養
支原體肉湯培養基(不含瓊脂和酚紅)配方:(g/L) | |||||||||||||||||
豬胃消化物 牛肉浸粉 酵母浸粉 氯化鈉 葡萄糖 pH值7.6 ± 0.2 | 10.0 5.0 5.0 2.5 5.0 25℃ | ||||||||||||||||
原理: | |||||||||||||||||
牛肉浸粉、酵母浸粉提供氮源、維生素和生長因子,氯化鈉維持均衡的滲透壓,葡萄糖提供碳源。 | |||||||||||||||||
用法: | |||||||||||||||||
稱取本品 27.5g,加熱溶解于 800ml 蒸餾水中,121℃高壓滅菌 15 分鐘,冷至室溫,無菌操作加入 滅活小牛血清或馬血清 200ml 和青霉素 80 萬單位,混勻,分裝無菌試管,備用。 | |||||||||||||||||
質量控制: | |||||||||||||||||
在36±1℃培養7天
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